anti rabbit cd31 platelet endothelial cell adhesion molecule  (Novus Biologicals)

Journal: Journal of animal science

Article Title: Dietary fiber supplementation during the last 50 days of gestation improves the farrowing performance of gilts by modulating insulin sensitivity, gut microbiota, and placental function.

doi: 10.1093/jas/skad021

Figure Lengend Snippet: Figure 4. Effects of konjac flour (KF) and combined dietary fiber (CDF) supplementation during the last 50 days of gestation on placental angiogenesis of gilts. (A) The hematoxylin and eosin methods were used to examine blood vessel density in the CON, KF, and CDF placental tissues, with black arrows for placental blood vessels (bar = 50 μm). (B) The percentage of blood vessels in the placental tissues. Each gilt was regarded as an experimental unit (n = 6 per group). (C) Western blot analysis of angiogenesis-related factors and protein expression in different placentae. (D) Protein expression levels of CD31 and VEGF-A. Each placenta was regarded as an experimental unit (n = 12 per group). a,bMean values with different small letters indicate a significant difference at P < 0.05.

Article Snippet: After blocking with TBS/T buffer containing 5% milk, the membranes were incubated with the primary antibodies against vascular endothelial growth factor A (VEGF-A) (19003-1-AP, Proteintech, USA, 1:1,000), platelet endothelial cell adhesion molecule (CD31) (A0378, Abclonal, China, 1:1,000), and β-actin (4970, CST, USA, 1:1,000).

Techniques: Western Blot, Expressing